Purification of beta-toxin from Clostridium perfringens type C.

نویسندگان

  • J Sakurai
  • C L Duncan
چکیده

Beta-toxin was purified about 340-fold from culture supernatant fluid of Clostridium perfringens type C with a yield of about 24% in terms of biologically active beta-toxin. The purification involved ammonium sulfate fractionation, gel filtration through Sephadex G-100, isoelectrofocusing in a pH 3 to 6 gradient, and immunoaffinity chromatography. The purified beta-toxin gave a single band on polyacrylamide gel electrophoresis.

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عنوان ژورنال:
  • Infection and immunity

دوره 18 3  شماره 

صفحات  -

تاریخ انتشار 1977